DRAWING OF CELL LINE
Celther Polska offers a cell line derivation service. Our advantage is the fast delivery time and competitive price. We have many years of experience in the implementation of stable cell lines. We make every effort to meet customer expectations.
The scope of services performed:
- Stable transfection of viral and plasmid vectors into cell lines
- Cloning and mutagenesis
- Cell selection (G418, neomycin, puromycin, etc)
- Isolation and screening of clones
- Identification, validation and banking of mobile phones
- Storage of cell lines in liquid nitrogen or -80 ° C
- Performing a mycoplasma test
- Sequencing by the Sanger method (Applied Biosystems® 3130 Genetic Analyzer) or NGS (Ion PGMTM System)
Our highly specialized staff works with modern laboratory equipment. In addition to the basic equipment, we also have an NGS sequencer – Ion PGM TM System (Ion Torrent®), BioStation CT (Nikon Corporation) – a device for real-time cell observation, and xCELLigence (Agilent Technologies, Inc.).
In addition, before starting the project implementation, we offer free consultations aimed at assessing the project and presenting specific solutions.
Derivation of human induced pluripotent stem cells (hiPSc) and their differentiation
The hiPSc derivation service includes:
- Derivation of hiPSc from the customer-supplied cellular model
- Derivation of hiPSc from the cellular model available in our laboratory
- Study of the expression of pluripotency markers
- Determination of the amount of mRNA
In addition, we provide the service of testing the pluripotency of stem cells provided by the client using techniques such as Western Blotting, immunocytochemistry or qPCR.
Derivation of spherical cell cultures
Celther offers the derivation of 3D spherical cell cultures that reflect in vivo conditions and are a suitable tool for pre-clinical testing.
